Guidelines

Guidelines for Facility Access, Sample Submission, and data management

 

Step 1: Initial consultation: Investigators should sign up for a consultation prior to sample submission to ensure a clear understanding of sample requirements for processing through the facility.  To arrange a consultation you can contact Drs. Wai Lam / Bin Deng by phone (802-656-4709, 802-656-5099) or email (ylam@uvm.edubdeng@uvm.edu).

 

If you need help please contact Dr. Bin Deng (802-656- 5099, bdeng@uvm.edu) or Dr. Wai Lam (802-656-4709, ylam@uvm.edu), or send an email to proteomics@uvm.edu.

  • For gel samples, please provide an image of the gel and mark the bands or spots that are to be analyzed. We need to be sure your samples and sample preparation methods are compatible with mass spectrometry and your sample has an adequate amount of the target proteins to be measured by mass spectrometry. Usually, a visible silver or coomassie stained gel band/spot that corresponds to 200 femtomole - 1 picomole of protein is needed to identify the protein.
  • For protein samples in solution, you must provide us the expected protein concentration and the composition of the solution.
  • Samples submitted for phosphorylation analysis must be Coomassie-stainable (in gel) or with a concentration higher than 5 pmol (in solution).

 

Step 2: Fill out the sample submission form and answer the questions listed. Each sample must be accompanied by a completed form. The information you provide helps to ensure proper handling of your sample.

Sample Submission Form

 

Step 3: Only trained persons directly operate mass spectrometers in the facility. However, the users are welcome to come, learn and participate in their sample analysis.

 

Step 4: Results will be sent via e-mail or provided as a printed copy. The raw data will be provided upon request.

It is best to send samples via FedEx if you are a non-UVM user or drop off samples in person. Please be advised that drop-off hours are Monday through Friday, 9:00 AM to 4:00 PM, and you must call us before dropping off. If you have any questions, please call us at 656- 5099 or email to proteomics@uvm.edu.

Download Protocols:

Investigators should use silver staining reagents that are compatable with mass spectrometry. The facility recommends SilverQuestTM(Invitrogen) and Focus FAST silver (G Biosciences, Cat # 786-240). However, investigators could also use mass-spec compatible reagents from other vendors.

Contact Information:

Vermont Genetics Network
University of Vermont
120A Marsh Life Science Building
Burlington, VT 05405-0086
(802) 656-4087
(802) 656-2914 - FAX
VGN@UVM.edu

Contact the Webmaster:
vgnwebstaff@list.uvm.edu

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