Proteomics Outreach
About us
The Proteomic Outreach project of the Vermont Genetics Network was made possible through a grant to the VGN funded by the NCRR and by a grant to Dr. Bryan Ballif funded by the NSF. The team developed a hands-on undergraduate proteomics laboratory module which was beta tested at UVM in the Spring of 2009 to take to undergraduate schools in Vermont. We completed our first outreach delivery to Saint Michael's College in the Fall of 2009 and to Johnson State College in the Spring of 2010. The module was then taught at Green Mountain College and Norwich University in the fall of 2010. It has been integrated into the curriculum at Saint Michael's College and was taught in the Spring of 2011.
Team Members:
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GOAL:
The goal of the VGN Proteomics Outreach project is to expose undergraduates in the state of Vermont to proteomics technology using hands-on laboratory experiences. We provide all teaching materials, laboratory materials and equipment for colleges within the state to run the module. All materials become the property of the recipient institution upon completion of the proteomics laboratory module. The undergraduate students learn about this cutting edge technology and gain new skills that we believe will help them with their future scientific careers.
OVERVIEW:
In this module, students will learn how protein expression in yeast is changed after exposure to oxidative stress or an environmental toxin. Total protein is then harvested and prepared for 2D gel analysis. Proteins with differential expression will be isolated from the 2D gel and prepared for Mass Spectrometry at the UVM Proteomics Core Facility. The data is processed and students examine their results and use bioinformatics tools to further understand the biological implications of the results.
MATERIALS:
Laboratory Manual
Proteomics Manual
- Click HERE to download doc.
- If you use this version to make edits make sure you save as a pdf for students to print out to maintain formatting.
- Click HERE to download pdf.
PowerPoints
- Day 1
- Day 2
- Day 3
- Day 4
- Day 6
Appendix Material
General Biology Review for DNA and RNA as well as gene and protein structure.- Day 1
- Day2
- Day 3
- Day 4
- Day 5
MSDS documents Click Here
References:
Questions about outreach? E-mail Janet.Murray@uvm.edu
