Microarray OutreachView all deployments by year About us The Microarray Outreach project of the Vermont Genetics Network was made possible through a grant supplement to the VGN grant funded by the NCRR. A Microarray Outreach Team was assembled in early fall of 2003. The team developed a hands-on undergraduate microarray laboratory module using the Affymetrix microarray platform to take to undergraduate schools in Vermont. The beta test of the module was run at the University of Vermont in the fall of 2003 and was subsequently delivered to all Baccalaureate Partner Institutions between 2004-2006 (St. Michael's College, Middlebury College, Johnson State College, Norwich University, and Castleton State College). Microarray Outreach has also expanded to non-baccalaureate partner institutes including Green Mountain College and Lyndon State College in the fall of 2005 and 2006 respectively and to Marlboro College in the spring of 2007. One of the goals of Microarray Outreach is the integration of this technology into the science curriculum at colleges throughout the state. We are seeing this goal being achieved with subsequent deliveries at several colleges including Johnson State College in the Spring of 2006, St. Michael's College and Middlebury College in the Spring of 2007, Norwich University and Green Mountain College in the fall of 2007 and Johnson State College, Castleton State College and Marlboro College in the Spring of 2008. Team Members:
The goal of the VGN Microarray Outreach project is to expose undergraduates in the state of Vermont to microarray technology using hands-on laboratory experiences. We provide all teaching materials, laboratory materials and equipment for colleges within the state to run the module. All materials become the property of the recipient institution upon completion of the microarray laboratory module. The undergraduate students learn about this cutting edge technology and gain new skills that we believe will help them with their future scientific careers. OVERVIEW: In this module, students will learn how gene expression in yeast is changed after exposure of yeast to dimethyl sulfoxide (DMSO), a common environmental contaminant and solvent. Control yeast are exposed to water and "treated" yeast are exposed to 10% DMSO for 2 hours. RNA is then harvested and prepared for use on Affymetrix® yeast microarray chips. Prepared cRNA is taken back to the UVM Microarray Core Facility and hybridized with the yeast chips. The data are processed using the most recent probeset signal intensity algorithms including RMA and GCRMA, the primary software utilized in visualizing gene expression differences is Genesifter. Students are then taken through basic microarray data analysis procedures to generate lists of genes which are up or down regulated upon treatment with DMSO. We have also developed a second experimental protocol inducing oxidative stress in the fission yeast S. pombe. This experimental protocol was beta-tested in the Biology Department at UVM in the Spring of 2007 and has been delivered at Green Mountain College and Castleton State College MATERIALS:Laboratory Manuals
S. cerevisiae (yeast)
S. pombe (fission yeast) "Global transcriptional responses of fission yeast to environmental stress" Mol Biol Cell. 2003 Jan;14 - FREE Pubmed Central full text (PMID: 12529438) PowerPoint Lecture Slides S. cerevisiae
S. pombe Other specific powerpoint LecturesMicroarray Useful Links:
Questions about the module? E-mail Janet.Murray@uvm.edu
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