Microarray Facility:
Sample submission, storage, and retrieval Policy
Qualitative Assessment using the Bioanalyzer
Samples brought to the microarray facility for total RNA, mRNA, cDNA, or DNA quality assessment should be in a rack or box labelled with PI name and placed in the -20 freezer labeled "Microarray" located in 305 HSRF. DO NOT BRING YOUR STOCKS. Please bring a 2ul aliquot in a 0.5 ml. microcentrifuge tube that is labeled on the top of the tube and corresponds exactly with what is written on the order form. Make sure RNA or DNA is at the proper concentration range for the chip analysis requested. All unused aliquots will be tossed away after the assessment is completed and the data uploaded to your BioDesktop account or emailed.
Microarray Target Preparation: RNA or DNA
Samples for target preps for new expression profiling projects should be submitted as 200ng total RNA at a concentration of 20ng/ul or greater (for lower concentrations see the Facility staff). Samples for ongoing expression studies using the standard Affymetrix target prep should be submitted as 3 ug of total RNA at a concentration >250ng/ul. Genomic DNA for mapping studies should be submitted as 400ng total DNA at a concentration of 40ng/ul or greater. It is critical that this DNA be resuspended in T10E0.1 (see staff if you need some of this reagent). Regardless of sample type, place samples with tops labeled in rack or box with PI name in the Microarray -20 freezer located in 305 HSRF. Make sure appropriate order forms are submitted and sample names are matched exactly between the order form and labeled tube. DO NOT SUBMIT YOUR STOCKS. RNA or DNA left over from target preps will be stored for six months and will be tossed by the facility staff at their discretion without prior notification.
